A constitutive promoter: DNA sequence from a subclover stunt virus.
Patent issued to CSIRO Division of Plant Industry,
Canberra, Australia (1998)
Technical expertise
Breeding techniques and genetic analysis
Protein characterization
Isolation and culture of protoplasts
Tissue culture for regenerating plants
Gene isolation and chimeric gene construction
Gene promoter analysis
Gene transfer to plants
Northern, Southern and Western blotting analyses
Pulse and chase technique
PCR, Cell-free synthesis of protein
Field-testing of genetically engineered plants.
Awards and Honors
Received New Zealand Genetical Society's Award of Excellence, 1988.
Commonwealth Scholarship (New Zealand Government) for Ph. D. study, 1984.
Bangladesh Agricultural University Merit Scholarship, for M.S. study, 1975.
University talentship, Bangladesh Agricultural University for B.S. study, 1970.
Education Boards' Scholarship (meritorious performance) for H.S.C.
study, 1968.
Employment (Past and Present)
Staff Scientist III (Sep 2004 to date)
Syngenta Biotechnology, Inc.
Transformation Technology Group, Soybean Transformation
Team.
Staff Scientist II (Mar 2002 to Aug 2004)
Syngenta Biotechnology, Inc.
Leader, Transformation Technology Group, Soybean
Transformation Team
(Epicotyl System)
Staff Scientist I (Mar 1999 to Feb 2002)
Syngenta Biotechnology, Inc.
Transformation Technology Group, Developed
transformation systems for bean
and sunflower.
Senior Scientist (Sep 1996 to Feb 1999)
University of Minnesota
Genetic engineering of soybean. Improvement of seed
protein quality of soybean
with a gene encoding sulphur-rich protein isolated from
sunflower.
Research Scientist (Jun 1989 to Aug 1996)
CSIRO, Division of Plant Industry, Canberra, Australia.
Development of Agrobacterium-mediated transformation
systems for
subterranean clover and jute. Improvement of economic
and agronomic value of
subterranean clover by inserting useful genes.
UNDP Consultant (1993)
Initiated a genetic engineering program on jute at the
Bangladesh Atomic Energy
Commission as an UNDP consultant. Conducted a course on
Molecular Biology.
Ph. D. student (1984-1988)
Botany Dept, Victoria University of Wellington, New
Zealand.
Tissue and Protoplast culture for improvement of
mungbean and Oca
(Oxalis tuberosa Mol).
Senior Scientific Officer (1982-1984)
Bangladesh Institute of Nuclear Agriculture.
Improvement of chickpea and lentil through ionizing
radiations as well as cross
breeding methods.
Senior Scientific Officer (Sugarcane Breeder) (1981-1982)
Bangladesh Sugarcane Research Institute, Ishurdi,
Bangladesh.
Breeding and genetic analysis of sugarcane.
Scientific Officer (1978-1981)
Bangladesh Institute of Nuclear Agriculture.
Breeding and genetic analysis of rice and tomato.
Lecturer (1977-1978)
Shere Bangla Agricultural University, Dhaka,
Bangladesh.
Botany Dept., Taught genetics courses to
3rd and 4th year B.Sc. students.
References
Dr. T. J. Higgins
CSIRO Division of Plant Industry, Canberra
GPO Box 1600, ACT - 2601, Australia.
Tel: 61-2-6246 5063. Fax: 61-2 6246 5000.
E.mail: tj.higgins@csiro.au
Dr. Serajul Islam
Adviser, Bangladesh Institute of Biotechnology
Savar, Dhaka, Bangladesh.
Tel. 800-2-9006035
Dr. Rouf Mian
Research Molecular Geneticist (USDA-ARS)
Adjunct Faculty, Horticulture and Crop Sciences
The Ohio State University
215-A Williams Hall, OARDC
1680 Madison Avenue, Wooster, Ohio 44691
Phone 330-263 3672.
Email: mian.3@osu.edu
Dr. Shib Sankar Basu
Syngenta Biotechnology, Inc.
3054 Cornwallis Road, RTP, NC 27709.
Tel. 919-541 8668, Fax. 919 541 8585
Email: shib.basu@syngenta.com
Dr. Manuel Sainz
Syngenta Biotechnology, Inc.
3054 Cornwallis Road, RTP, NC 27709.
Tel. 919-541 8505, Fax. 919 541 8585
Email:
manuel.sainz@syngenta.com
Publications
Published
18 Research papers in peer-reviewed journals, presented 14 papers in
International conferences and authored two books.
Research Articles:
Wenck, A., Pugiex, C., Turner, M., Dunn, M., Stacy, C., Tiozzo, A., Dunder,
E., Van-Grinsvan, E., Khan, R., Sigareva, M., Wang,
W-C., Reed, J., Drayton,
P., Oliver, D., Trafford, H., Legris, G., Rushton, H.,
Tayeb, S., Launis,
K., Chang, Y-F., Chen, D-F., Melchers, L. (2003)
Reef-coral proteins as
visual, non-destructive reporters for plant
transformation. Plant Cell Reports
22(4): 244-251.
Dear, B.S., Sandral, G.A., Spencer, D., Khan, M.R.I., Higgins, T.J.V. (2003)
The tolerance of three transgenic subterranean clover
(Trifolium subterraneum L.)
lines with bxn gene to herbicides containing
bromoxynil. Australian Journal of
Agricultural Research. 54(2): 203-210.
Ke, J., Khan, R., Johnson, T., Somers, D.A. and Das, A (2001) High efficiency
gene transfer to recalcitrant plants by Agrobacterium
tumefaciens. Plant Cell
Reports. 20:150-156.
Chu, P.W.G., Anderson, B.J., Khan, M.R.I., Shukla, D., Higgins, T.J.V. (1999)
Production of bean yellow mosaic virus resistant
subterranean clover (Trifolium
subterraneum). Ann. Appl. Biology. 135(2):480-489.
Tabe, L., Molvig, L., Khan, R., Schroeder, H., Gollasch, S.,
Wardley-Richardson,
T., Moore, A., Craig, S., Spencer, D., Eggum, B.,
Higgins, T.J.V. (1997)
Modifying the sulphur amino acid content of protein in
transgenic legumes.
In: Sulphur Metabolism in Higher plants (Ed. W.J. Cram
et al.). Backhuys
Publishers. The Netherlands. pp. 87-93.
Khan, M.R.I., Ceriotti, A., Tabe, L.M., Aryan, A., McNabb, W., Moore, A.,
Craig,
S., Spencer, D., Higgins, T.J.V. (1996) Accumulation of
a sulfur-rich seed
albumin from sunflower in the leaves of transgenic
subterranean clover (Trifolium
subterraneum L.) Transgenic Research 5:179-185.
Khan, M.R.I., Tabe, L.M., Heath, L.C., Spencer, D., Higgins, T.J.V. (1994)
Agrobacterium-mediated transformation of subterranean
clover. Plant
Physiology 105:81-88.
Wandelt, C. I., Khan, M.R.I., Craig, S., Schroeder, H., and Higgins, T.J.V.
(1992)
Vicilin with carboxy-terminal KDEL is retained in the
endoplasmic reticulum and
accumulated to high levels in the leaves of transgenic
plants. The Plant Journal.
2:181-192.
Wandelt, C., Knibb, W., Schroeder, H. E., Khan, M. R. I., Spencer, D., Craig,
S.,
Higgins, T. J. V. (1991) The expression of an ovalbumin
and a seed protein gene
in the leaves of transgenic plants. Plant Molecular
Biology 2. (Eds. R. Herman
and B. Larkins) Plenum Press, New York. p.p. 471-478.
Schroeder, H., Khan, M.R.I., Knibb, W. R., Spencer, D., Higgins, T. J. V.
(1991)
The expression of a chicken ovalbumin gene in three
Lucerne cultivars. Aust. J.
Plant Physiol. 18:495-5056.
Khan, M.R.I., Heyes, J.K. and Cohen, D. (1988) Plant regeneration from oca
(Oxalis tuberosa): The effect of explant type and
culture media. Plant Cell Tissue
and Organ Culture. 14: 41-50.
Khan, M.R.I., Heyes, J.K. and Cohen, D. (1987) Regeneration and somaclonal
variability in oca (Oxalis tuberosa). Proc.
International Congress of Plant Tissue
Culture, Bogota, Colombia. September 21-26, 1987.
Shaikh, M.A.Q., Khan, M.R.I., Shamsuzzaman, K.M., and Bhuiya, A.D. (1983)
Variability induction in chickpea through sodium azide
treatment. Mutation
Breeding Newslatter. 22: 9-10.
Hossain, M.A., Khan, M.R.I. and Shaikh, M.A.Q. (1982) Morphological and
genetic variation in the hybrid derivatives of Oxheart
and Anobic tomato cross.
Bangladesh Journal of Botany. 11 (1):37-54.
Khan, M.R.I., Khanum, S., Shaikh, M.A.Q. and Kaul, A.K. (1981) Genetic studies
in tomato (Lycopersicon esculentum Mill). Bangladesh
Journal of Botany.
10 (2): 157-163.
Khan, M.R.I., Gatehouse, J.A. and Boulter, D. (1980) The seed proteins of
cowpea (Vigna unguiculata L. Walp). Journal of
Experimental Botany (U.K.).
31:1599-1611.
Kaul, A.K., Khan, M.R.I., Choudhury, M.H. and Shaikh, M.A.Q. (1978) Nutritional
and cooking quality characters of some local rice
cultivars of Bangladesh. Proc.
Symp. Seed Protein Improvement by Nuclear Techniques.
IAEA, Vienna.
p. 167-179.
Manuscripts under preparation
Khan, M.R.I. and Higgins, T.J.V (2006) Agrobacterium-mediated transformation of
jute (Corchorus capsularis L.)
Khan, M.R.I., Somers, D. and Higgins, T.J.V. (2006) Stable expression of a
sulphur-rich protein gene from sunflower in
soybean seeds.
Books
Kaul, A.K., Khan, M.R.I. and Munir, K.M. (1992) Rice quality: a survey of
Bangladesh germplasm. Bangladesh Rice Research
Institute, Dhaka.
Pages. 1-178.
Khan, M.R.I. and Podder, B.P. (1992) Sugarcane varieties in Bangladesh.
Bangladesh sugarcane Research Institute.
Pages. 1-129.
Presentations at Scientific
Meetings and Conferences
Ke, J., Khan, R.I., Somers, D.A., Das, A. (1998) An improved method for the
high
efficiency transformation of plants by Agrobacterium
tumefaciens. In: 19th Annual
Crown Gall Meeting, Nov. 7-8, 1998. Purdue University,
West Lafayette, IN, USA.
Khan, M.R.I., Donovan, C.M., Zhang, Z., Clemente, T.E., Higgins, T.J., and
Somers, D.A., (1998) Genetic engineering of a sunflower
sulfur-rich protein gene
into soybean. 7th Biennial Conference on the Molecular
and Cellular Biology of
the Soybean, Knoxville, Tennessee, 26-29 July, 1998.
Islam, M.S., Ahmed, G., Hossain, A.B.M. , Khan, R.I. (1996) Genetic
transformation of jute: Progress and Prospects. In:
Support for Varietal
Improvement of Jute and Kenaf. 5-13 August, 1996.
International Jute
organisation, Dhaka, Bangladesh.
Khan, M.R.I., Spencer, D., Leigh, J., Moore, A., Holgate, M., Higgins, T.J.V.
(1995) Field trial of genetically engineered
subterranean clover containing genes
for sulphur-rich protein and herbicide resistance. 2nd
International Plant Tissue
Culture Conference, Dhaka University, 17 -19 December,
1995, Dhaka,
Bangladesh.
Leigh, J., Spencer, D., Khan, M.R.I., Moore, A., Holgate, M., Higgins, T.J.V.
(1995) Planned release of genetically engineered
subterranean clover containing
genes for sulphur rich protein and herbicide
resistance. 35th Annual General
Meeting of the Australian Society of Plant
Physiologists, Sept. 26-29, 1995,
Sydney, Australia.
Khan, M.R.I., Tabe, L., Aryan, A., Ceriotti, A., Craig, S., Higgins, T.J.V.
(1994)
Expression of a sunflower seed albumin gene in
subterranean clover. 34th Annual
General Meeting of the Australian Society of Plant
Physiologists. Sept. 27-30,
1994. Broadbeach, Queensland.
Khan, M.R.I., Heath, L.C., Spencer, D., Higgins, T.J.V (1993) Expression of
foreign genes in subterranean clover. 33rd Annual
General Conference of the
Australian Society of Plant Physiologists. University
of Western Australia. 26-31
September, 1993.
Khan, M.R.I., Heath, L.C., Spencer, D., Higgins, T.J.V (1992) Agrobacterium-
mediated transformation of subterranean clover.
Australian Society of Plant
Physiologists. La Trobe university, Australia. 29
September - 3 October, 1992.
Wandelt, C. I., Khan, M. R. I., Craig, S., Spencer, D., and Higgins, T. J. V.,
ER-
retention signal enhances the expressioin of a pea
vicilin gene. International
symposium on plant Molecular Biology & Biotechnology.
24-26 September,
1990, Australian Society of Plant Physiologists,
Sydney, Australia.
Khan, M. R. I., Heyes, J.K. and Cohen, D., Variability induction in oca (Oxalis
tuberosa Mol) by tissue culture: selection of better
yielding types. Genetics and
Tissue Culture Conference, (29 August - 2 September,
1988), Lincoln University,
New Zealand.
Khan, M. R. I., Heyes, J.K. and Cohen, D., Regeneration and somaclonal
variability in oca (Oxalis tuberosa Mol). International
Congress of Plant Tissue
Culture, Bogota, Colombia, September 21-26, 1987.
Khan, M. R. I. and Heyes, J. K., Tissue culture of mungbean (Phaseolus aureus
Roxb). Sixth Biennial Conference of the New Zealand
Branch of the International
Association of Plant Tissue Culture, Massey University,
New Zealand,
February 12-13, 1985.
Khan, M. R. I. and Heyes, J. K., Plant regeneration from petiole and internode
sections of oca (Oxalis tuberosa Mol). Sixth Biennial
Conference of the New
Zealand Branch of the International Association of
Plant Tissue Culture, Massey
University, New Zealand, February 12-13, 1985.
Khan, M. R. I., Research for nutritional improvement in Bangladesh. In a
conference on 'Basic Needs' organized by the 'Overseas
Development Institute' at
the University of Birmingham (U.K.), April
7, 1980.